Figure 6. Stimulating effects of 2-hydroxyethyl methacrylate (2-HEMA),2-hydroxyethyl acrylate (2-HEA), ethyl acrylate (EA), triethylenglycoldimethacrylate (TEGDMA) and methyl methacrylate (MMA) on hemeoxygenase 1 (Ho1) mRNA expression in RAW264.7 cells. The cells wereincubated for 3.5 h with each acrylate-related compound at aconcentration of 10-1,000 μM, and then their total RNAs were prepared.Each cDNA was synthesized, and the expression level of Ho1 mRNA wasdetermined by real-time polymerase chain reaction and standardizedagainst the expression of 18s rRNA. The results are presented asmeans±standard error (SE) of three independent experiments, SE<15%.*Significantly different at p<0.01 vs. control group.