Figure 7. Effects of trans-cinnamic acid (CA), trans-cinnamaldehyde(CMA) and methyl cinnamate (MC) on Porphyromonas gingivalislipopolysaccharide (LPS)-stimulated cyclo-oxygenase-2 (Cox2), nitricoxide synthase 2 (Nos2) and tumor necrosis factor-alpha (Tnfa) mRNAexpression in RAW264.7 cells. The cells were pretreated for 30 min withthe indicated doses of compounds, then they were incubated for 3 h withor without P. gingivalis LPS at 100 ng/ml, and their total RNAs wereprepared. Each cDNA was synthesized, and the expression level of eachmRNA was determined by real-time polymerase chain reaction andstandardized against the expression of 18s rRNA. The results are presentedas means±standard error (SE) of three independent experiments, SE<15%.*Significantly different at p<0.01 vs. LPS-treated group.