Figure 8. Effects of 2-hydroxyethyl methacrylate (2-HEMA), 2-hydroxyethylacrylate (2-HEA), ethyl acrylate (EA), triethylenglycol dimethacrylate(TEGDMA) and methyl methacrylate (MMA) on Porphyromonas gingivalislipopolysaccharide (LPS)-stimulated cyclo-oxygenase-2 (Cox2), nitric oxidesynthase 2 (Nos2) and tumor necrosis factor-alpha (Tnfa) mRNA expressionin RAW264.7 cells. The cells were pretreated for 30 min with the indicateddoses of compounds, they were then incubated for 3 h with or without P.gingivalis LPS at 100 ng/ml, and their total RNAs were prepared. EachcDNA was synthesized, and the expression level of each mRNA wasdetermined by real-time polymerase chain reaction and standardizedagainst the expression of 18s rRNA. The results are presented asmeans±standard error (SE) of three independent experiments, SE<15%.*Significantly different at p<0.01 vs. LPS-treated group.