Figure 1. Effects of PMSG and hCG injections on the ovaries of prepubertal mice at 21 days of age. A: The total numbers of antral follicles andcorpora lutea (CLs) in ovaries from prepubertal mice were counted at 0 and 48 h after PMSG injection and 4, 8, 12, 24 and 48 h after hCG injection,respectively. B: Follicle stage index, preovulatory (>390 μm), large antral (250-390 μm) and small antral follicles (140-250 μm). C-F: HE stainingof 21-day-old mouse ovaries at 0 h (C) and 48 h after PMSG (D), 12 h (E) and 48 h (F) after hCG in PMSG-primed mice. Bar=200 μm. Differentletters (a, b and c) on A and B indicate significant changes through the experiment (p<0.05). S: Small antral follicle; L: large antral follicle; Pre:preovulatory follicle, CL: corpus luteum. n=5 in each group.