Figure 9. Time course changes in the expression of genes involved in luteinization (Sfrp4, Ptgs2, Tnfaip6) and EGF-like ligands (Areg, Ereg, Btc, Egfr)in ovaries from control and PCOS-model mice at 0, 24, 48, and 56 h after PMSG treatment and 2, 4 and 8 h after hCG treatment. Data were expressedrelative to mRNA expression in the ovaries at diestrus (=1.0). Different letters (a, b, c, d and e) indicate significant changes in the expression of eachgene (p<0.05). Straight line and dotted line indicate with and without hCG treatment in PMSG-primed mice at 48 h, respectively. n=5 in each group.