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. 2018 Dec 21;41(3):1638–1648. doi: 10.3892/or.2018.6948

Figure 5.

Figure 5.

miR-498 inhibits ZEB2 expression in liver cancer cells. (A) TargetScan software prediction of miR-498 downstream genes. The seed sequences of miR-498 in the wild type (WT) or mutant type (MUT) of ZEB2 3′-UTR are indicated. (B) The WT or MUT ZEB2 3′-UTR were cloned into luciferase reporter vector. The luciferase activity in 293T cells transfected with the WT (or MUT) ZEB2 vector and miR-498. (C) qRT-PCR and (D) western blot analyses of ZEB2 expression levels in HepG2 cells transfected with miR-498. (E) Western blot analyses showed decreased expression of β-catenin and c-Myc in miR-498-transfected HepG2 cells. (F) Western blot analyses showed decreased expression of TGF-β, p-SMAD2 and p-SMAD3 in miR-498-transfected HepG2 cells. **P<0.01, ***P<0.001. ZEB2, zinc finger E-box binding homeobox 2.