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. 2018 Dec 21;41(3):1638–1648. doi: 10.3892/or.2018.6948

Table I.

Primers used for qRT-PCR.

Gene Sequence (5′-3′) Product size (bp) Annealing temperature (°C)
E-cadherin F: 5′-CGCATTGCCACATACACTCT-3′ 252 55
R: 5′-TTGGCTGAGGATGGTGTAAG-3′
N-cadherin F: 5′-AGTCAACTGCAACCGTGTCT-3′ 337 55
R: 5′-AGCGTTCCTGTTCCACTCAT-3′
Vimentin F: 5′-GAGCTGCAGGAGCTGAATG-3′ 344 55
R: 5′- AGGTCAAGACGTGCCAGAG-3′
Slug F: 5′-CCTGGTTGCTTCAAGGACAC-3′ 395 55
R: 5′-TCCATGCTCTTGCAGCTCTC-3′
Twist F: 5′-ACGAGCTGGACTCCAAGATG-3′ 484 55
R: 5′-GGCACGACCTCTTGAGAATG-3′
Snail F: 5′-GCGAGCTGCAGGACTCTAAT-3′ 310 55
R: 5′-GCCTCCAAGGAAGAGACTGA-3′
Bcl-2 F: 5′-GGATCCAGGATAACGGAGGC-3′ 150 55
R: 5′-CCAGATAGGCACCCAGGGT-3′
Cyclin D1 F: 5′-CCGAGAAGCTGTGCATCTAC-3′ 221 55
R: 5′-CTTCACATCTGTGGCACAGAG-3′

F, forward; R, reverse.