Table I.
Gene | Sequence (5′-3′) | Product size (bp) | Annealing temperature (°C) |
---|---|---|---|
E-cadherin | F: 5′-CGCATTGCCACATACACTCT-3′ | 252 | 55 |
R: 5′-TTGGCTGAGGATGGTGTAAG-3′ | |||
N-cadherin | F: 5′-AGTCAACTGCAACCGTGTCT-3′ | 337 | 55 |
R: 5′-AGCGTTCCTGTTCCACTCAT-3′ | |||
Vimentin | F: 5′-GAGCTGCAGGAGCTGAATG-3′ | 344 | 55 |
R: 5′- AGGTCAAGACGTGCCAGAG-3′ | |||
Slug | F: 5′-CCTGGTTGCTTCAAGGACAC-3′ | 395 | 55 |
R: 5′-TCCATGCTCTTGCAGCTCTC-3′ | |||
Twist | F: 5′-ACGAGCTGGACTCCAAGATG-3′ | 484 | 55 |
R: 5′-GGCACGACCTCTTGAGAATG-3′ | |||
Snail | F: 5′-GCGAGCTGCAGGACTCTAAT-3′ | 310 | 55 |
R: 5′-GCCTCCAAGGAAGAGACTGA-3′ | |||
Bcl-2 | F: 5′-GGATCCAGGATAACGGAGGC-3′ | 150 | 55 |
R: 5′-CCAGATAGGCACCCAGGGT-3′ | |||
Cyclin D1 | F: 5′-CCGAGAAGCTGTGCATCTAC-3′ | 221 | 55 |
R: 5′-CTTCACATCTGTGGCACAGAG-3′ |
F, forward; R, reverse.