FIG 5.

The presentation of major surface polysaccharides by P. gingivalis W83 is not altered by growth in the presence of galactose. Cultures of P. gingivalis W83 were grown in TSBHK alone or TSBHK supplemented with 0.5%, 0.1%, 0.05%, or 0.01% galactose (gal) for 24 h. Before generating autoclaved extracts, a fixed volume of the cultures was removed (A) or the cultures were normalized to an OD₆₀₀ of 1.0 (B), and ELISAs were performed to detect capsular polysaccharides (A and B). The extracts generated using a fixed volume of culture as in panel A were used in subsequent ELISAs to detect the O-LPS (C) and A-LPS (D).