TABLE 2.
Bacterial orders significantly affected by at least one dietary treatment in the rumen of nonlactating cows fed methane-reducing additivesc
|
Bacterial order |
Relative abundance (%) |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Study 1a |
Effect |
Study 2b |
Effect |
|||||||||||||
| CTL | LIN | NIT | LIN+NIT | SEM | Linseed | Nitrate | lin×nit | CTL | TEA | NIT | TEA+NIT | SEM | Saponin | Nitrate | sap×nit | |
| Bacteroidales | 46.5 | 44.0 | 46.5 | 44.0 | 0.010 | 0.88 | 0.54 | 0.35 | 39.98 | 37.55 | 45.92 | 42.18 | 0.012 | 0.12 | 0.01 | 0.76 |
| Selenomonadales | 1.54 | 2.27 | 1.77 | 2.76 | 0.002 | 0.03 | 0.42 | 0.82 | 2.14 | 1.95 | 2.11 | 2.66 | 0.001 | 0.40 | 0.14 | 0.11 |
| Coriobacteriales | 0.29 | 0.20 | 0.39 | 0.36 | 0.000 | 0.16 | 0.01 | 0.39 | 0.22 | 0.19 | 0.32 | 0.37 | 0.000 | 0.73 | 0.00 | 0.18 |
| Gastranaerophilales | 0.18 | 0.17 | 0.07 | 0.07 | 0.000 | 0.99 | 0.09 | 0.83 | 0.26 | 0.21 | 0.11 | 0.10 | 0.000 | 0.34 | 0.00 | 0.57 |
| Unclassified | 0.14 | 0.20 | 0.14 | 0.05 | 0.000 | 0.30 | 0.02 | 0.03 | 0.17 | 0.18 | 0.14 | 0.11 | 0.000 | 0.59 | 0.10 | 0.47 |
| Synergistales | 0.05 | 0.06 | 0.03 | 0.06 | 0.000 | 0.04 | 0.26 | 0.26 | 0.05 | 0.03 | 0.05 | 0.03 | 0.000 | 0.07 | 0.96 | 0.60 |
| Elusimicrobiales | 0.02 | 0.10 | 0.06 | 0.08 | 0.000 | 0.04 | 0.56 | 0.22 | 0.03 | 0.04 | 0.07 | 0.04 | 0.000 | 0.71 | 0.87 | 0.46 |
| Burkholderiales | 0.01 | 0.01 | 0.06 | 0.06 | 0.000 | 0.82 | 0.00 | 0.62 | 0.01 | 0.01 | 0.11 | 0.04 | 0.000 | 0.29 | 0.01 | 0.44 |
| Unclassified Deltaproteobacteria (×10−3) | 7.08 | 1.42 | 2.03 | 2.66 | 0.000 | 0.09 | 0.26 | 0.15 | 4.36 | 0.31 | 2.97 | 1.00 | 0.000 | 0.03 | 0.93 | 0.43 |
| Victivallales (×10−3) | 6.44 | 4.65 | 1.09 | 6.17 | 0.000 | 0.28 | 0.27 | 0.04 | 1.38 | 3.44 | 1.54 | 4.18 | 0.000 | 0.12 | 0.97 | 0.92 |
| Xanthomonadales (×10−3) | 4.68 | 2.37 | 3.64 | 8.64 | 0.000 | 0.98 | 0.45 | 0.48 | 7.74 | 1.28 | 2.77 | 9.45 | 0.000 | 0.99 | 0.37 | 0.00 |
| Micrococcales (×10−3) | 4.26 | 13.5 | 5.16 | 10.2 | 0.000 | 0.01 | 0.59 | 0.65 | 8.22 | 6.51 | 15.9 | 9.85 | 0.000 | 0.19 | 0.13 | 0.62 |
| Opitutae vadin HA64 (×10−3) | 0.36 | 7.24 | 1.53 | 3.94 | 0.000 | 0.06 | 0.78 | 0.31 | 0.68 | 1.16 | 3.14 | - | 0.000 | 0.40 | 0.97 | 0.14 |
In study 1, cows were fed a control (CTL) diet and CTL diet supplemented with linseed (LIN), nitrate (NIT) and linseed plus nitrate (LIN+NIT). Tested effects were linseed (CTL and NIT versus LIN and LIN+NIT) and nitrate (CTL and LIN versus NIT and LIN+NIT) and their interaction, lin×nit.
In study 2, cows were fed a control (CTL) diet and control diet supplemented with tea saponin (TEA), nitrate (NIT) and tea saponin plus nitrate (TEA+NIT). Tested effects were saponin (CTL and NIT versus TEA and TEA+NIT) and nitrate (CTL and TEA versus NIT and TEA+NIT) and their interaction, sap×nit.
Values are the means from four observations, and analysis was performed on square root-transformed taxonomic tables using the aov function in R.