TABLE 1.
Strains and plasmids used and constructed in this study
| Strain or plasmid | Origin or genetic features | Source or reference |
|---|---|---|
| P. yayanosii strains | ||
| A1 | Facultative piezophile, derivative strain of P. yayanosii CH1 | 20 |
| A1/pLMOS776TA | A1 with plasmid pLMOS776TA | This study |
| A1/pLMOS812TA | A1 with plasmid pLMOS812TA | This study |
| A1/pLMOS839TA | A1 with plasmid pLMOS839TA | This study |
| A1/pLMOS1206TA | A1 with plasmid pLMOS1206TA | This study |
| Δ1369 mutant | A1 with ΔPYCH_13690 | This study |
| Plasmids | ||
| pLMO12102 | Shuttle plasmid replicated in both P. abyssi GE5 and E. coli | X. Ma, unpublished data |
| pLMOS01 | pLMO12102::Pgdh-HMG-CoA from P. furiosus | This study |
| pLMOS011 | pLMO12102::Phhp1-HMG-CoA from P. furiosus | This study |
| pLMOS012 | pLMO12102::Phhp2-HMG-CoA from P. furiosus | This study |
| pLMOS776TA | pLMO12102::HMG-CoA-Pgdh-PhmbB-PF0775-PF0776-Phhp1 | This study |
| pLMOS812TA | pLMO12102::HMG-CoA-Pgdh-PhmbB-PF0813-PF0812-Phhp1 | This study |
| pLMOS839TA | pLMO12102::HMG-CoA-Pgdh-PhmbB-PF0883-PF0839-Phhp1 | This study |
| pLMOS1206TA | pLMO12102::HMG-CoA-Pgdh-PhmbB-PF1207-PF1206-Phhp1 | This study |
| pLMOS507Tpy | pLMO12102::Pgdh-HMG-CoA-Phhp1-PYCH05070 | This study |
| pLMOS1312Tpy | pLMO12102::Pgdh-HMG-CoA-Phhp1-PYCH13120 | This study |
| pLMOS1772Tpy | pLMO12102::Pgdh-HMG-CoA-Phhp1-PYCH17720 | This study |
| pLMOS944Tpf | pLMO12102::Pgdh-HMG-CoA-Phhp1-PF0944 | This study |