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. 2019 Jan 31;7:15. doi: 10.3389/fchem.2019.00015

Figure 6.

Figure 6

Spiropyrazoline oxindole 1a reduces stemness of GL-261 mouse glioma cells while sensitizes conventional chemotherapeutic-induced cell death. GL-261 cells were treated with compound 1a or DMSO (control) for 24 h and collected for MTS and Western blot analysis. (A) Cell viability as analyzed by MTS metabolism in GL-261 cells treated with 5, 12.5, or 25 μM of compound 1a. (B) Representative immunoblots (top) and corresponding densitometry analysis (bottom) of Sox2 and β-III tubulin protein levels after 24 h of 12.5 or 25 μM of 1a treatment. Ponceau staining was used as loading control. (C) Cell viability as analyzed by MTS metabolism in GL-261 cells co-treated with 1a (12.5 μM) and the chemotherapeutic agent TMZ (400 μM) for 24 h. Data represent mean ± SEM of at least three independent experiments. *p < 0.05, **p < 0.005, and ****p < 0.0001 from DMSO; ±p < 0.01 and §p < 0.0001 from TMZ-treated cells; ±±p < 0.0001 from 1a-treated cells by one-way ANOVA followed by Tukey's multiple comparison test. TMZ, temozolomide.