Figure 10.

(A) Random surface-initiated copolymerization of an azide-containing SBMA monomer and a nonfunctional SBMA monomer (q = 0, 1, or 5%), followed by a SPAAC reaction of the azides to immobilize biotin inside the brush. (B) Protein adsorption and binding onto bare and polymer-brush-coated (as described in A) surfaces as measured by reflectometry: Top, fibrinogen adsorption; middle, avidin adsorption; bottom, bar plot summarizing fibrinogen adsorption, the avidin binding of pure avidin, and a mixed protein solution of avidin and fibrinogen.⁷³