Figure 5.

Co-culture Organoids Grown from Benign and Cancer Regions of the Same Patient Maintain Histologic Phenotype and Improve Passaging

(A) Isolation and co-culture of matched primary prostate organoids derived from benign and cancer regions of the same patient, with PrS cells.

(B) Matched benign (PrE-5) and cancer (PrECa-5) tissue from the patient from which cells were isolated for organoid growth. Histology shown by hematoxylin and eosin staining (top panel), and presence of AMACR shown by immunohistochemistry (bottom panel). 10X image shown with 40X inset for AMACR.

(C) Matched organoids derived from benign regions (PrE-5) and cancer regions (PrECa-5) from patient tissue shown in (B) grown in mono-culture and co-culture in 3D with PrS mix 5. Histology shown by hematoxylin and eosin staining (top panel), and presence of AMACR shown by immunohistochemistry (bottom panel). 40X images shown with 100-μM scale bar.

(D) Whole-well images of passage 0 and passage 2 mono- and co-culture PrECa-7 organoids derived from cancer regions. High magnification of selected organoid shown in inset. Inset shown with 100-μm scale bar.

(E) Expression of AMACR by qRT-PCR in total RNA isolated from matched organoids derived from benign and cancer tissue shown in (C) and passage 2 organoids shown in (D). Note that the benign organoids did not survive passage.

p value < 0.1 was considered statistically significant. *p < 0.1, **p < 0.05, ***p < 0.01, ****p < 0.001; ns, not significant.