Fig. 5.

Digoxin inhibited Nrf2 by decreasing Nrf2 mRNA at transcriptional level in SW1990/Gem and Panc-1/Gem cells. (A–D) Quantitative real-time PCR analysis. (E–F) SW1990/Gem and Panc-1/Gem cells were treated with 80 nM of digoxin, 20 µM of MG132, or a combination of digoxin and MG132 for 6 h, the protein level of Nrf2 were detected by Western blot. (G–H) CHX-chase analysis. (I–K) Ubiquitination assay. Data were expressed as mean ± SD, and the results were representative of three independent experiments. Significant differences were indicated as **P < 0.01, ***P < 0.001 vs. control group, ^###P < 0.001 vs. MG132 group. N.S., no significant.