UNBS5162 induces SKOV3 cell apoptosis. (A) SKOV3 cells, treated with UNBS5162 for 24 h, were labelled with annexin V/FITC and PI and analysed by flow cytometry. The proportion of apoptotic cells was determined. (B) Western blot analysis of SKOV3 cells treated with UNBS5162 for 24 h. The band intensities were quantified relative to tubulin expression. *P<0.05 vs. NC group. NC, negative control; PI, propidium iodide; FITC, fluorescein isothiocyanate; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein.