Colony forming ability and cell viability of non-small cell lung cancer cells were decreased following HMGB3 knockdown. (A) Typical result of colony forming assay showing a decreased colony forming ability of A549 or H1299 cells following HMGB3 knockdown. (B) The CFE of A549 cells was decreased following HMGB3 knockdown. (C) Cell viability of A549 or H1299 was decreased following HMGB3 knockdown. Data in (B and C) are from three independent repeats. Statistical significance test was performed on data obtained on the last time point. ****P<0.0001. shRNA, short hairpin RNA; HMGB3, high mobility group box 3; NT, non-transfected control; shHMGB3, shRNA targeting HMGB3; shNC, negative control shRNA; OD, optical density; CFE, colony formation efficiency.