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. 2019 Jan 31;5:346. doi: 10.3389/fvets.2018.00346

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Copyright © 2019 Mühleip, Lin and Kraiczy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Binding of polymorphic FH to viable LD spirochetes. Indicated Borrelia strains were incubated in human serum as well as in mouse, rat, dog, cat, rabbit, cattle, horse, or chicken serum. Following serum incubation, spirochetes were washed extensively, and surface-bound proteins were eluted using 0.1 M glycine (pH 2.0). Both, the last wash (W) and the eluate (E) fractions obtained from each reaction were separated by glycine-SDS-PAGE under non-reducing conditions and transferred to nitrocellulose. FH molecules were detected by using a sheep anti-FH antibody (1:500). Purified human FH (500 ng) served as a control in all tests. The mobility of the molecular mass standard is indicated on the left of each panel.