Figure 3.

Identification of CRASP involved in binding of polymorphic FH molecules. Borrelia strains ectopically producing CspA_B31, CspA_PKo, CspA_A14S, CspZ, ErpC, or ErpP were incubated in human serum and various animal sera. Following incubation, spirochetes were washed extensively and surface-bound proteins were eluted using 0.1 M glycine (pH 2.0). Both, the last wash (W) and the eluate (E) fractions obtained from each reaction were separated by glycine-SDS-PAGE under non-reducing conditions and transferred to nitrocellulose. FH molecules were detected by using a sheep anti-FH antibody (1:500). Purified human FH (500 ng) served as a control. The mobility of the molecular mass standard is indicated on the left of each panel.