Table 1.
Genes within the hot plate QTL on chromosome 7 that have a cis-eQTL associated with rs3148686.
| Chr | Gene | Name | Start (Mb) | Dist. (Mb) | Cod. | N.Cod. | P value | FDR | FC H | FC N | Pain | Inflam. | 129P2? |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 7 | Ryr1 | Ryanodine receptor 1 | 29 | 1.18 | 0 | 9 | 1.22E-07 | 1.50E-05 | 1.25 | 1.02 | Yes | Yes | Yes |
| 7 | Erf | ETS2 repressor factor | 25.24 | 5.06 | 0 | 0 | 2.98E-06 | 1.80E-04 | 1.23 | 1.14 | No | No | No |
| 7 | Cyp2a5 | Cytochrome P450 family 2 subfamily A member 5 | 26.84 | 3.46 | 0 | 0 | 3.16E-05 | 1.00E-03 | 2.79 | 3.82 | Yes | Yes | No |
| 7 | Rps5 | Ribosomal protein S5 | 12.92 | 17.38 | 0 | 2 | 1.20E-04 | 0.0027 | −1 | 1.09 | No | Yes | Yes |
| 7 | Gys1 | Glycogen synthase 1 | 45.44 | 15.13 | 0 | 0 | 2.20E-04 | 0.0044 | −1.17 | −1.26 | Yes | Yes | No |
| 7 | Rps11 | Ribosomal protein S11 | 45.12 | 14.82 | 0 | 0 | 4.90E-04 | 0.0081 | 1.02 | 1.09 | No | No | No |
| 7 | Cox6b1 | Cytochrome C oxidase subunit 6B1 | 30.62 | 3.1 | 0 | 1 | 5.00E-04 | 0.0081 | 1.04 | 1.19 | No | No | No |
| 7 | Pou2f2 | POU class 2 homeobox 2 | 25.09 | 5.17 | 0 | 6 | 1.00E-03 | 0.014 | 1.1 | −1.06 | Yes | Yes | Yes |
| 7 | Clip3 | CAP-Glydomain containing linker protein 3 | 30.29 | 0.19 | 0 | 2 | 1.30E-03 | 0.017 | −1.07 | −1.04 | No | Yes | Yes |
| 7 | Sirt2 | Sirtuin 2 | 28.77 | 1.52 | 0 | 2 | 2.30E-03 | 0.025 | −1.12 | −1.06 | Yes | Yes | No |
| 7 | Lmtk3 | Lemur tyrosine kinase 3 | 45.78 | 15.48 | 0 | 0 | 2.50E-03 | 0.026 | 1.16 | 1.09 | No | No | No |
| 7 | Actn4 | Actinin alpha 4 | 28.89 | 1.34 | 0 | 3 | 3.70E-03 | 0.034 | 1.1 | 1.12 | Yes | Yes | No |
| 7 | Syt3 | Synaptotagmin 3 | 44.38 | 14.08 | 0 | 0 | 4.60E-03 | 0.04 | 1.15 | 1.14 | No | No | No |
| 7 | Ltbp4 | Latent transforming growth factor beta binding protein 4 | 27.31 | 2.97 | 5'UTR | 1 | 0.0052 | 0.044 | 1.21 | 1.14 | No | Yes | Yes |
| 7 | Cyp2g1 | Cytochrome P450 family 2 subfamily G member 1 | 26.81 | 3.49 | 0 | 0 | 0.0053 | 0.044 | 2.15 | 3.55 | No | No | No |
Dist.(Mb): distance of gene from the SNP rs3148686 (30.31 Mb), the SNP nearest the QTL for hot plate sensitivity; Cod.: coding polymorphisms; N.Cod.: non-coding polymorphisms; FDR: false discovery rate; FC H: fold-change in gene expression in mice heterozygous (H) for the rs3148686 marker relative to mice homozygous for the B6J (B) allele; FC N: fold-change in gene expression in mice homozygous for the B6NJ allele (N) relative to mice homozygous for the B allele; Pain: pain literature search (“gene” and “pain” or “nociception” PubMed); Inflam.: inflammation literature search (“gene” and “inflammation” or “inflammatory” PubMed). 129P2?: whether or not the gene contains a variant shared between the nociception-resistant B6NJ strain and the 129P2 strain; QTL: quantitative trait locus.