Figure 3.

miR-544 inhibits metastasis and epithelial-mesenchymal transition of anaplastic thyroid cancer cells via the expression of YY1. SW1736 cells were transfected with miR-NC, miR-544 mimic or miR-544 mimic and YY1 plasmid, while 8305C cells were transfected with anti-miR-NC, miR-544 inhibitor or miR-544 inhibitor and si-YY1 for 48 h prior to analysis. (A) wound-healing assay was used to analyze the migration capability of SW1736 cells. (B) Wound-healing assay was used to analyze the migration capability of 8305C cells. Transwell migration and Matrigel invasion assays were used to detect the migration and invasion capability, respectively. (C) Images (captured at ×100 magnification) of migration and invasion of SW1736 cells in the lower chamber (scale bars, 50 µm). (D) Quantification of migration and invasion of SW1736 cells in the lower chamber (scale bars, 50 µm). (E) ages (captured at ×100 magnification) of migration and invasion of 8305C cells in the lower chamber (scale bars, 50 µm). (F) Quantification of migration and invasion of 8305C cells in the lower chamber (scale bars, 50 µm). (G) The protein expression of E-cadherin and N-cadherin in SW1736 cells was detected with a western blot analysis assay (H) The protein expression of E-cadherin and N-cadherin in 8305C cells was detected with a western blot analysis assay. *P<0.05. YY1, Yin Yang-1; miR, microRNA; miR-NC, miR-negative control; anti-miR-NC, inhibitor control.