Fig. 3.

DiPaC of the sod BGC into pET28b-ptetO::sod_gfpv2. a PCR amplification of the 4.6 kb sodA–D fragment with Q5 polymerase and primers containing homology sequences for SLIC into the backbone, b sodA–D fragment after gel purification, c PCR amplification to construct a linear 6.6 kb backbone of pET28b-ptetO::gfpv2, d positive screening PCR of five clones after transformation of the SLIC-joined pET28b-ptetO::sod_gfpv2 plasmid and e control restriction digest of two clones with NdeI or EcoRV in comparison to the predicted (Snapgene) pattern