Fig. 5.

ACY-1215 and ACY-214 alter TIL phenotype. TILs harvested from resected melanoma tumors were expanded with 500nM ACY-1215 or DMSO. (a) CD8+ and CD4+ T-cells central memory percentages were assessed in six patient samples across four experiments based on CD45RO + CD62L+, CD45RA-CD62L+, CD45RA-CCR7+ or CD45RO + CD45RA-CD62L+ gating strategies. (b) After expansion, TILs were washed, activated and evaluated for the percent of CD45RO + CD62L+ or CD45RA-CD62L+ subsets. Data are from six patient samples assessed across four experiments. (c) TILs expanded with 500nM ACY-1215, 500nM ACY-241 or DMSO were stimulated with PMA/Ionomycin and monensin-treated for four hours. Intracellular IFNγ and CD107a expression was assessed by flow cytometry. Representative contour plots of CD8+ TILs from one out of four patient samples assessed are shown. (d) Paired analysis of DMSO versus ACY-1215 treated CD8+ IFNγ+CD107a + TILs are shown for all four patients assessed across three experiments. *p < 0.05