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. 2019 Feb 6;19:8. doi: 10.1186/s12898-019-0224-1

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Biological impact of marbled crayfish on Madagascar. a Real-time PCR detection of the crayfish plague agent Aphanomyces astacii in animals that were collected in various locations in Madagascar. Numbers of animals analyzed per site are indicated by circle size (small = 10, intermediate = 20, large = 30). Agent levels are indicated by section colors. b Sympatry of marbled crayfish and Astacoides betsileoensis in the Andragnaroa river. c Laboratory experiment to test the predation of Biomphalaria pfeifferi snails by marbled crayfish. Bar: 2 cm. d Leftover B. pfeifferi shells, found after overnight co-housing with marbled crayfish. Bar: 2 mm. Map and pictures were produced by the first author