. 2019 Feb 7;10:10. doi: 10.1186/s40104-019-0318-9

Table 4.

Effect of dietary L-lysine·H₂SO₄ levels on serum amino acid concentrations of broilers ¹

Items	Dietary L-lysine·H₂SO₄ levels, %					SEM	P-value
Items	0	1	4	7	10	SEM	ANOVA	Linear	Quadratic
Essential AA, nmol/mL
Lysine	402.0^c	576.5 ^c	1031.4^b	1395.3^a	1521.8^a	105.18	< 0.01	< 0.01	< 0.01
Arginine	374.9	310.3	281.7	319.4	284.4	43.55	0.58	0.21	0.37
Histidine	114.9	145.6	137.3	144.7	157.7	14.91	0.36	0.07	0.20
Isoleucine	112. 9^b	170.9^ab	189.0^ab	231.9^a	156.4^ab	21.39	< 0.01	0.10	< 0.01
Leucine	175.8^b	258.0^ab	229.0^ab	283.0^a	228.3^ab	24.59	0.04	0.12	0.04
Methionine	68.9	80.8	79.4	81.5	76.7	6.93	0.69	0.44	0.37
Phenylalanine	121.5^b	166.7^a	178.8^a	176.5^a	138.7^ab	11.96	< 0.01	0.30	< 0.01
Threonine	623.5^b	667.7^b	776.7^b	1070.5^b	1453.0^a	117.63	< 0.01	< 0.01	< 0.01
Valine	169.4^c	260.9^b	312.9^b	408.6^a	310.1^b	27.71	< 0.01	< 0.01	< 0.01
Non-essential AA, nmol/mL
Alanine	1043.5^b	1797.6^ab	806.1^b	2167.4^a	848.9^b	258.68	< 0.01	0.92	0.23
Asparagine	79.9^b	182.1^ab	118.8^ab	221.3^a	143.4^ab	30.28	0.02	0.11	0.07
Glycine	764.9^b	1058.2^a	918.5^ab	911.0^ab	845.5^ab	66.07	0.05	0.95	0.09
Cysteine	304.9	403.0	296.5	415.1	238.6	53.94	0.12	0.57	0.22
Proline	1926.8^ab	2622.0^a	1464.6^b	1881.1^ab	2415.6^a	204.41	< 0.01	0.80	0.50
Serine	647.1	764.4	601.3	656.5	751.3	58.40	0.27	0.63	0.71
Tyrosine	109.6	153.1	137.4	137.9	143.2	12.26	0.17	0.21	0.21

¹Means in the same row with different superscripts significantly differ from one another (P < 0.05). AA amino acid, SEM standard error of the mean; n = 6/diet