Table 1.
Details of the qPCR/PCR assays used in the study for the detection of pathogen DNA in flea samples
| Target species (gene) | PCR primer and probe sequences (5'-3') | Product size (bp) | Reference |
|---|---|---|---|
| Flea (18S rRNA) | 18S-F: GATCGTACCCACATTACTTG | 1200 | [27] |
| 18S-R: AAAGAGCTCTCAATCTGTCA | |||
| Dipyllidium caninum (28S rRNA) | F: GCATGCAAGTCAAAGGGTCCTACG | 653 | [48] |
| R: CACATTCAACGCCCGACTCCTGTAG | |||
| Bartonella spp. (ssrA) | F: GCTATGGTAATAAATGGACAATGAAATAA | 299 | [28]a |
| R: GGCTTCTGTTGCCAGGTG | |||
| FAM-ACCCCGCTTAAACCTGCGACG-BHQ1 | |||
| Mycoplasma haemofelis (16S rRNA) | F: GTGCTACAATGGCGAACACA | 80 | [29] |
| R: TCCTATCCGAACTGAGACGAA | |||
| FAM-TGTGTTGCAAACCAGCGATGGT-BHQ1 | |||
| “Candidatus Mycoplasma haemominutum” (16S rRNA) | F: TGATCTATTGTKAAAGGCACTTGCT | 135 | [29] |
| R: TTAGCCTCYGGTGTTCCTCAA | |||
| FAM-TTCAATGTGTAGCGGTGGAATGCGT-BHQ1 | |||
| “Candidatus Mycoplasma turicensis” (16S rRNA) | F: AGAGGCGAAGGCGAAAACT | 138 | [29] |
| R: ACGTAAGCTACAACGCCGAAA | |||
| FAM-CGTAAACGATGGGTATTAGATGTCGGGAT-BHQ1 |
aThe reverse primer has been modified compared to the one described in the paper