Fig. 4.

Inhibiting arginase activity has therapeutic efficacy in Kras mutant Gemms by creating an immune favorable microenvironment. a Representative MRI image of Kras mutant mouse lung tumors from mice treated with Compound 9 for one week. b Quantification of MRI images of lung tumors of mice either treated with vehicle or compound 9 for up to four weeks, each dot represents an individual mouse. p values for each of the 1,2 and 4 week time points are indicated on the graph c Measurement of arginine levels in the lung tumor tissue and blood of mice either treated with vehicle or compound 9 by metabolomics. d % CD3, CD4, and CD8 T cells in all CD45 cells, and e CD8 to regulatory T cell (FoxP3+) ratios in the lung tumor microenvironment of mice treated with vehicle or compound 9. determined by multicolor flow cytometry analysis. f Quantification of interferon gamma production by flow cytometry of ex vivo stimulated and enriched lymphocytes from the lung tumors in mice treated with vehicle or compound 9 for one week. All p values in this figure were determined by Student’s t test. Each data point in the graphs is from a different mouse