Fig. 5.

ChIP analysis reveals ESR1 and JUN, but not FOS, bind to promoter I.f in N42 hypothalamic cells. We performed ChIP assays using N42 hypothalamic cell extracts treated with vehicle or 10⁻⁷ M E₂ for 6 h. Sonicated cell supernatant was used as input DNA (positive control). Precleared chromatin was used for IP reactions with a rabbit polyclonal antibody directed against human (A) ESR1 and (B) JUN or FOS, as well as normal rabbit IgG. ESR1 was recruited to the E₂-responsive nt −193/−47 but not to a more distal (nt −925/−768) region (see Fig. 4). Estradiol induced the recruitment of JUN but not FOS to the nt −193/−47 region. The figures represent one of three independently performed experiments.