Table 1.
The qRT-PCR and amplification PCR primer sequences of the goat PDGFRB gene. Note: TD, touch-down PCR.
| primer name | primer sequences (5′-3′) | sizes (bp) | detection methods |
|---|---|---|---|
| PDGFRB-F | GAGTCGGTGGACTATGTGCC | 189 | qRT-PCR |
| PDGFRB-R | CTGGTAGCTGAAGCCCACAA | ||
| Stra8-F | TCTCACACTCCTCCGTCACT | 192 | qRT-PCR |
| Stra8-R | ATGCCTGCAAGAGGATGGTC | ||
| GAPDH-F | AAAGTGGACATCGTCGCCAT | 116 | internal control |
| GAPDH-R | CCGTTCTCTGCCTTGACTGT | ||
| P1(PDGFRB-1F) | CCAGCTCAGGATGGGTCT | 296 | TD |
| P1(PDGFRB-1R) | CCAGCATGGGCACATAGTC | ||
| P2(PDGFRB-2F) | ACCTGAATCTGTCTGGTGTGT | 222 | TD |
| P2(PDGFRB-2R) | CAGAAAGGGAAAGGGACATGC | ||
| P3(PDGFRB-3F) | GCTGGGTGAGGGCTACAAAA | 120 | TD |
| P3(PDGFRB-3R) | AAACACCAGTGCGTCACAGT |