Figure 5. A high tumor clone frequency in skin is associated with a distinct gene expression profile and a higher number of somatic mutations.

A. Unsupervised analysis by hierarchical clustering (complete linkage) according to the expression of 78 genes in 157 patients reveals 3 different clusters of patients. Intensity expression values in the heatmap are expressed as log2 fold changes compared to the average expression of each gene in the whole study group. The tumor clone frequency in each sample is represented by a colour scale at the bottom of the heatmap. B. Dot plots of the T cell percentages of nucleated cells) in patients in cluster 1, 2 and 3. Medians were compared by Mann-Whitney U-test with Bonferroni adjustment for multiple testing, * p<0.05 **p<0.01 C. Dot plots of the tumor clone frequency (TCF) in patients in cluster 1, 2 and 3. Means were compared by Mann-Whitney U-test with Bonferroni adjustment for multiple testing, * p<0.05 **p<0.01 D. Kaplan-Meier estimates of progression-free survival in 157 patients with cutaneous T cell lymphomas in the training group, according to the gene expression clustering. Log-rank test with Bonferroni adjustment for multiple testing, * p<0.05 **p<0.01 ***p<0.001 E. Whole exome sequencing data of microdissected skin T cells in patients with mycosis fungoides. Number of somatic mutations according to the clinical stage (left). Mann-Whitney U-test, * p<0.05 Number of somatic mutations according to the malignant clone frequency in skin (right). Spearman correlation, p<0.05 considered significant