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. Author manuscript; available in PMC: 2019 Feb 7.
Published in final edited form as: Physiol Genomics. 2006 Mar 28;25(3):502–513. doi: 10.1152/physiolgenomics.00321.2005

Fig. 6.

Fig. 6.

Development of virus-induced MCM and AHR in mClca3−/− mice. Both mClca3−/− and wild-type control mice were inoculated with SeV or SeV-UV and analyzed 21 days later follows. A: lung RNA was subjected to real-time PCR for mRNA levels of mClca3 and Muc5ac normalized to the Gapdh control. Values represent means ± SE for 3–5 mice. B: corresponding lung sections were subjected to immunostaining with anti-hCLCA1/mClca3 Ab and Alexa 633-conjugated secondary Ab (green) as well as biotinylated anti-Muc5ac Ab and Alexa 555-streptavidin (red) and counterstained with Sytox green (blue). Bar = 20 μm. C: quantitative analysis of immunostaining from B. D: AHR to inhaled MCH was assessed using Penh measurements as described in Fig. 1. Values represent means ± SE for 8 mice. *Significant differences from SeV-UV in A, C, and D.