Fig 4.

(A) miR-34A promoter methylation in choroid plexus carcinomas (n = 42). The miR-34A gene structure is depicted, with the promoter CpG island as the gray block. Methylation levels are measured by bisulfite pyrosequencing at three adjacent CpG sites. (B) Kaplan-Meier curve of overall survival in a patient with choroid plexus carcinoma; survival stratified by miR-34A tumor methylation levels. Clinical data were available for 29 patients, and overall survival is shown for patients with miR-34A hypermethylated tumors (n = 16) and miR-34A hypomethylated tumors (n = 13). P = .0331 between the two groups by log-rank (Mantel-Cox) test.