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. Author manuscript; available in PMC: 2019 Feb 7.
Published in final edited form as: Methods Mol Biol. 2013;1042:3–12. doi: 10.1007/978-1-62703-526-2_1

Fig. 3.

Fig. 3

Controls used in screen. (a) Upon treatment of cells with RU486 or dexamethasone (DEX), GR translocates into the nucleus. However, the fraction of arrays formed by GFP–GR binding to the MMTV repeats is lower in cells treated with RU486 than cells treated with DEX. (b) Immunofluorescence for GAPDH in cells transfected with nontargeting siRNA or cells transfected with siGAPDH. (c) Quantification of total cell intensity for GAPDH immunofluorescence. Numbers are an average from six 96-well plates with four wells per plate containing either nontargeting siRNA or siRNA to GAPDH