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. 2019 Feb 6;34(1):613–619. doi: 10.1080/14756366.2019.1569647

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Effect of NCT and NCPA on the expression of immunoregulatory cytokines in the ear skin tissues (a) and CD4⁺ T cells in draining lymph nodes (b). The ear skin tissues were excised on day 28 and total RNA was isolated. Quantitative real-time polymerase chain reaction was performed as described in the Methods. Data are presented as the mean ± SD of triplicate determinations. *indicates significant difference from the value of the DFE/DNCB-treated mice at p < 0.05. AD was induced by DFE and DNCB treatment. DNCB: 2,4-dinitrochlorobenzene; DFE: Dermatophagoides farina extract; AD: atopic dermatitis. Means denoted with different letters (a–d) within each graph are significantly different from each other at p < 0.05.