KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
| Antibodies | ||
| FITC anti-mouse CD45 (clone 30-F11) | BioLegend | Cat# 103108; RRID: AB_312973 |
| FITC anti-human CD45 (clone HI30) | BioLegend | Cat# 304006; RRID: AB_314394 |
| PerCP/Cy5.5 anti-mouse TCR β chain (clone H57–597) | BioLegend | Cat# 109228; RRID: AB_1575173 |
| APC/Cy7 anti-mouse CD4 (clone RM4–5) | BioLegend | Cat# 100526; RRID: AB_312727 |
| Alexa Fluor 700 anti-mouse CD8a (clone 53–6.7) | BioLegend | Cat# 100730; RRID: AB_493703 |
| Brilliant Violet 605 anti-mouse/human CD44 (clone IM7) | BioLegend | Cat# 103047; RRID: AB_2562451 |
| Brilliant Violet 711 anti-mouse CD62L (clone MEL-14) | BioLegend | Cat# 104445; RRID: AB_2564215 |
| PE anti-mouse IFN-γ (clone XMG1.2) | BioLegend | Cat# 505808; RRID: AB_315402 |
| APC anti-mouse TNF-α (clone MP6-XT22) | BioLegend | Cat# 506308; RRID: AB_315429 |
| PE/Cy7 anti-mouse CD11c (clone N418) | BioLegend | Cat# 117318; RRID: AB_493568 |
| Brilliant Violet 650 anti-human CD11c (clone 3.9) | BioLegend | Cat# 301638; RRID: AB_2563797 |
| APC/Cy7 anti-mouse I-A/I-E (clone M5/114.15.2) | BioLegend | Cat# 107628; RRID: AB_2069377 |
| PE anti-mouse CD80 (clone 16–10A1) | BioLegend | Cat# 104708; RRID: AB_313129 |
| Pacific Blue anti-mouse CD86 (clone GL-1) | BioLegend | Cat# 105022; RRID: AB_313145 |
| Brilliant Violet 605 anti-mouse CD103 (clone 2E7) | BioLegend | Cat# 121433; RRID: AB_2629724 |
| Brilliant Violet 650 anti-mouse/human CD11b (clone M1/70) | BioLegend | Cat# 101228; RRID: AB_893232 |
| APC anti-mouse Ly-6C (clone HK1.4) | BioLegend | Cat# 128015; RRID: AB_1732087 |
| Pacific Blue anti-mouse Ly-6G (clone 1A8) | BioLegend | Cat#127612; RRID: AB_2251161 |
| APC/Cy7 anti-human HLA-DR (clone L243) | BioLegend | Cat# 307618; RRID: AB_493586 |
| PE anti-mouse/human phospho-TBK1 (Ser172) (clone D52C2) | Cell Signaling Tech. | Cat# 13498S |
| PE anti-mouse/human phospho-TBK1 (Ser172) (clone J133–587) | BD Biosciences | Cat # 558604; RRID: AB_647214 |
| Alexa Fluor 647 anti-mouse/human phospho-IRF-3 (Ser396) (clone D6O1M) |
Cell Signaling Tech. | Cat# 10327S |
| PD-L1 antibody (10F.9G2) | BioLegend | Cat# 124321; RRID: AB_2563635 |
| InVivomAb anti-mouse CD8a neutralizing antibody (clone YTS 169.4) |
BioXcell | Cat# BE0117 |
|
InVivomAb anti-mouse IFNAR-1 neutralizing antibody (clone MAR1–5A3) |
BioXcell | Cat# BE0241 |
| Anti-PD-1 antibody (clone, 332.8H3) | Dr. Gordon Freeman's lab at Dana-Farber Cancer Institute |
N/A |
| Bacterial and Virus Strains | ||
| Ad5CMVCre | University of Iowa | VVC-U of Iowa-5 |
| Ad5CMVCre-eGFP | University of Iowa | VVC-U of Iowa-1174 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Olaparib | MedChem Express | Cat# HY-10162 |
| BX-795 hydrochloride | Sigma Aldrich | Cat# SML0694 |
| (2-Hydroxypropyl)-β-cyclodextrin (HPCD) | MedChem Express | Cat# HY-101103 |
| D-Luciferin, Potassium Salt | Gold BioTechnology | Cat# LUCK |
| Paraformaldehyde solution 4% in PBS | Santa Cruz | Cat# sc-281692 |
| 7-AAD Viability Staining Solution | BioLegend | Cat# 420404 |
| FITC Annexin V | BioLegend | Cat# 640906 |
| CDK1 inhibitor IV, RO-3306 | Calbiochem | Cat# 217699 |
| Mouse Recombinant GM-CSF | StemCell Technologies, Inc. | Cat #78017.1 |
| Collagenase/hyaluronidase | StemCell Technologies, Inc. | Cat# 07912 |
| DNase I | StemCell Technologies, Inc. | Cat# 07900 |
| Critical Commercial Assays | ||
| Quant-iT PicoGreen dsDNA Reagent | Life Technologies | Cat# P7581 |
| Mouse IFN beta ELISA Kit | Thermo Fisher Scientific | Cat# EN424001 |
| Annexin V Binding Buffer | BioLegend | Cat# 422201 |
| MEGM Bullet Kit | Lonza | Cat# CC-3150 |
| Dynabeads Mouse T-Activator CD3/CD28 for T Cell Expansion and Activation |
GIBCO | Cat#11452D |
| CellTrace Violet Cell Proliferation Kit, for flow cytometry | Life Technologies | Cat# C34557 |
| Deposited Data | ||
| Transcriptome data | GEO | GSE120500 |
| Experimental Models: Cell Lines | ||
| UWB1.289 | American Type Culture Collection | Cat# CRL-2945; RRID: CVCL_B079 |
| UWB1.289+BRCA1 | American Type Culture Collection | Cat# CRL-2946; RRID: CVCL_B078 |
| NHDC-Human Dendritic Cells | Lonza | Cat# CC-2701 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J-Tmem173 gt/J | The Jackson Laboratory | Stock No: 017537 |
| FVB/NJ mice | The Jackson Laboratory | Stock No: 001800 |
| C57BL/6J mice | The Jackson Laboratory | Stock No: 000664 |
| Brca1loxP/loxP mice | Dr. Jos Jonkers's laboratory | N/A |
| Trp53loxP/loxP mice | National Cancer Institute Mouse Repository |
N/A |
| PtenloxP/loxP mice | Dr. Hong Wu's laboratory | N/A |
| PBM | This paper | FVB/NJ |
| PPM | This paper | FVB/NJ |
| Recombinant DNA | ||
| BRCA1 Double Nickase Plasmid (m) | Santa Cruz Biotechnology | Cat# SC-419362-NIC |
| Software and Algorithms | ||
| PRISM 7 software | Graphpad | https://www.graphpad.com/scientific-software/prism/ |
| GSEA (v2.2.2) | Broad Institute | http://software.broadinstitute.org/gsea/index.jsp |
| Flowjo (version 10.1) | FlowJo, LLC | https://www.flowjo.com/solutions/flowjo/ |