Fig. 2.

miR-34c inhibited Dicer/Pten DKO cancer cell growth by inducing cell cycle arrest in G1 phase and apoptosis. Dicer/Pten cancer cell lines were transfected with mmu-miR-34c, mmu-let-7b, or miRNA mimic negative control using Lipofectamine RNAiMAX. After 2 days, viability of treated Dicer/Pten DKO cancer cells was measured by Promega CellTiter-Glo assay (A). Representative cell cycle profile of miR-34c-treated cells was determined by flow cytometry using PI staining and analyzed by flowjo software (B). Caspase activities of miR-34c-treated DKO-1 were measured by Promega caspase-Glo 3/7 assay (C). Data of cell viability, cell cycle profile, and caspase activity are expressed as mean values ± SEM for three determinations (*P < 0.05).