Table 1.
miR-34c regulated key mediators in cell proliferation and cell cycle G1/S transition.
| Genea | Complex | Fold change (miR-34c/miR-Ctrl) | Fold change (DKO mouse early tumor/normal FL) | Algorithmsb | |
|---|---|---|---|---|---|
| mRNA microarray | QPCR validation | ||||
| Orc6l* | Prereplication complex | −2.4 | −5.9 | 1.8 | M |
| Cdt1 | −3.2 | −6.3 | 6.3 | ||
| Cdc6* | −2.6 | −5.5 | 5.8 | M | |
| Mcm2 | −2.3 | −3.0 | 4.7 | ||
| Mcm3 | −2.4 | −4.9 | 4.4 | ||
| Mcm5* | −9.7 | −18.7 | 12.3 | M | |
| Mcm6 | −2.4 | −3.5 | 8.2 | ||
| Mcm7 | −2.8 | −4.2 | 5.1 | ||
| Mcm10 | −3.4 | −6.0 | 13.3 | ||
| Rfc1* | DNA polymerase complex | −3.3 | −3.3 | 1.4 | M |
| Rfc2* | −2.0 | −2.4 | 1.2 | M | |
| Rfc4 | −2.2 | −4.3 | 3.4 | ||
| Rfc5 | −2.3 | −5.3 | 2.2 | ||
| Top2a | −3.7 | −11.7 | 21.3 | ||
| Pold1 | −2.1 | −3.4 | 2.4 | ||
| Pold2 | −2.9 | −4.6 | 1.3 | ||
| Ccne2* | Cyclin/cyclin-depedent kinase complex | −2.6 | −4.6 | 3.5 | M, T |
| Ccnd3 | −2.5 | −2.9 | 2.9 | ||
| Cdk2 | −2.1 | −3.4 | 1.9 | ||
| Cdkn1c | 8.9 | 18.3 | −2.4 | ||
a
Of 20 genes, 19 were downregulated, of which 6 are predicted miR-34c targets.
b
Prediction algorithms: FL, fallopian tube; M, miRanda; T, TargetScan.
*
Genes with predicted miR-34c binding sites in their 3′ UTR sequences.