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Quantitative real-time PCR analysis of selected genes for RNA-Seq validation (experiment 2). Across 20 comparisons (10 genes × 2 heifer groups [pregnant vs. cyclic]), the expression pattern of the selected genes obtained by qPCR was consistent with the results from the RNA-Seq analysis in all but two cases (QRFPR and CCL20). For each transcript, bars with different superscripts differ significantly (P < 0.05). PII, pregnant ipsilateral isthmus; CII, cyclic ipsilateral isthmus.
