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Quantitative real-time PCR analysis of differentially expressed transcripts from experiment 2 in tissues from experiment 1. Across 20 comparisons (10 genes × 2 heifer groups [pregnant vs. cyclic]), the expression pattern of the selected genes was as expected, with no differences between groups in all but one case (KERA). For each transcript, bars with different superscripts differ significantly (P < 0.01). PII, pregnant ipsilateral isthmus; CII, cyclic ipsilateral isthmus.
