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Effects of anti-TLR4 Ab on cytotrophoblast proinflammatory cytokine production following LPS exposure. Isolated cytotrophoblast cells were pretreated with anti-TLR4 Ab for 2 h before the addition of PBS or 100 ng/ml LPS for 24 h. The levels of TNF-α (A), IL-1β (B), and IL-6 (C) in the culture supernatants were measured using ELISA. Data are presented as mean ± SD of four independent experiments.
