Table 1.
Antibody | Fluorochrome | Titer | Isotype | Manufacturer |
---|---|---|---|---|
Anti-CD45 Ab | Allophycocyanin (APC)/Cy7 | 1:5 | Mouse IgG1, κ | BioLegend |
Anti-CD163 Ab | PE/Cy7 | 1:10 | Mouse IgG1, κ | BioLegend |
Anti-vimentin Ab | Alexa Fluor 488 | 1:10 | Mouse IgG1, κ | BD Biosciences |
Anti-cytokeratin-7 Ab | Alexa Fluor 568 | 1:25 | Rabbit IgG | Abcam |
Anti-CD80 Ab | Fluorescein isothiocyanate (FITC) | 1:5 | Mouse IgG1, κ | BioLegend |
Anti-CD86 Ab | Peridinin chlorophyll (PerCp)/Cy5.5 | 1:5 | Mouse IgG2b, κ | BioLegend |
Anti-CD206 | APC/Cy7 | 1:5 | Mouse IgG1, κ | BioLegend |
Anti-CD209 Ab | PE/Cy7 | 1:5 | Mouse IgG2a, κ | BioLegend |
Anti-TNF-α Ab | APC | 1:5 | Mouse IgG1, κ | BioLegend |
Anti-IL-12p40 Ab | APC | 1:5 | Mouse IgG1, κ | BioLegend |
Anti-IL-10 Ab | PE | 1:5 | Rat IgG2a, κ | BioLegend |