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. 2016 Feb 1;94(2):33, 1-9. doi: 10.1095/biolreprod.115.134627

Table 1.

Antibodies used in the flow cytometry.

Antibody Fluorochrome Titer Isotype Manufacturer
Anti-CD45 Ab Allophycocyanin (APC)/Cy7 1:5 Mouse IgG1, κ BioLegend
Anti-CD163 Ab PE/Cy7 1:10 Mouse IgG1, κ BioLegend
Anti-vimentin Ab Alexa Fluor 488 1:10 Mouse IgG1, κ BD Biosciences
Anti-cytokeratin-7 Ab Alexa Fluor 568 1:25 Rabbit IgG Abcam
Anti-CD80 Ab Fluorescein isothiocyanate (FITC) 1:5 Mouse IgG1, κ BioLegend
Anti-CD86 Ab Peridinin chlorophyll (PerCp)/Cy5.5 1:5 Mouse IgG2b, κ BioLegend
Anti-CD206 APC/Cy7 1:5 Mouse IgG1, κ BioLegend
Anti-CD209 Ab PE/Cy7 1:5 Mouse IgG2a, κ BioLegend
Anti-TNF-α Ab APC 1:5 Mouse IgG1, κ BioLegend
Anti-IL-12p40 Ab APC 1:5 Mouse IgG1, κ BioLegend
Anti-IL-10 Ab PE 1:5 Rat IgG2a, κ BioLegend