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. 2018 Mar 26;159(5):2110–2126. doi: 10.1210/en.2017-03150

Figure 1.

Figure 1.

Resistance to thermic depression in fasting GLUT8-deficient mice. (A) Indirect calorimetry measurements of heat production, VO2, VCO2, and RER in WT and G8KO mice over a 48-hour period of ad libitum chow feeding or fasting (n = 4 to 6 mice per group). (B) ELISA quantification of serum free triiodothyronine (T3) hormone levels in WT and G8KO mice that were chow fed or fasted for 12 or 24 hours (n = 4 mice per group). (C) qRT-PCR quantification of GLUT8 mRNA in primary murine hepatocytes transfected with either scrambled ASOs (ASOScr) or sequence 1 (ASO1GLUT8) or 2 (ASO2GLUT8) of ASOs targeting GLUT8 (n = 3 replicates per group). (D) Indirect calorimetry measurements of heat production and VO2 in WT mice treated with ASOScr, ASO1GLUT8, or ASO2GLUT8 and either chow fed or fasted for 48 hours (n = 6 mice per group). Data are presented as mean ± standard error of the mean. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.