Table 1.
Mouse qRT-PCR Primer Sequences
| Gene Symbol | Protein Name | Forward Primer (5′–3′) | Reverse Primer (5′–3′) |
|---|---|---|---|
| Slc2a8 | GLUT8 | TTCATGGCCTTTCTAGTGACC | GAGTCCTGCCTTTAGTCTCAG |
| Slc2a2 | GLUT2 | TGTGCTGCTGGATAAATTCGCCTG | AACCATGAACCAAGGGATTGGACC |
| mt-Cytb | Cytochrome b | CTGTTCGCAGTCATAGCC | AAGAATCGGGTCAAGGTG |
| mt-Co1 | Cytochrome c oxidase I | ATGTTCTATCAATGGGAGC | TCTGAGTAGCGTCGTGGT |
| mt-Atp8 | ATP synthase 8 | ATGCCACAACTAGATACAT | TAGTGATTTTGGTGAAGG |
| mitochondrial d-loop | N/A | AATCAATGGTTCAGGTCA | ACGGAGGATGGTAGATTA |
| Actb | β-Actin | GGCTGTATTCCCCTCCATCG | CCAGTTGGTAACAATGCCATGT |
| Hmgcs2 | 3-Hydroxy-3-methylglutaryl-Coenzyme A synthase 2 | ATACCACCAACGCCTGTTATG | CAATGTCACCACAGACCACCA |
| Acox1 | Acyl-Coenzyme A oxidase 1 | CCTGATTCAGCAAGGTAGGG | TCGCAGACCCTGAAGAAATC |
| Cpt1a | Carnitine palmitoyltransferase 1a | AGTGGCCTCACAGACTCCAG | GCCCATGTTGTACAGCTTCC |
| Fgf21 | Fibroblast growth factor 21 (FGF21) | TACACAGATGACGACCAAGA | GGCTTCAGACTGGTACACAT |
| Atf4 | Activating transcription factor 4 | AGCAAAACAAGACAGCAGCC | ACTCTCTTCTTCCCCCTTGC |
| Tfam | Transcription factor A, mitochondrial | GCCTGAGTTTGTGTTTGCTG | CTGCTCTTTATACTTGCTCACAG |
| Nrf1 | Nuclear respiratory factor 1 | AATGTCCGCAGTGATGTCC | GCCTGAGTTTGTGTTTGCTG |
| Cox5b | Cytochrome c oxidase subunit Vb | ACCCTAATCTAGTCCCGTCC | CAGCCAAAACCAGATGACAG |
| Ndufs8 | NADH dehydrogenase (ubiquinone) Fe-S protein 8 | GTTCATAGGGTCAGAGGTCAAG | TCCATTAAGATGTCCTGTGCG |
| Uqcrc1 | Ubiquinol-cytochrome c reductase core protein I | ATCAAGGCACTGTCCAAGG | TCATTTTCCTGCATCTCCCG |
| rplp0 | 36B4/ ribosomal protein, large, P0 | TAAAGACTGGAGACAAGGTG | GTGTACTCAGTCTCCACAGA |
| Actin | Actin | GATTACTGCTCTGGCTCCTAG | GACTCATCGTACTCCTGCTTG |
Abbreviations: ATP, adenosine triphosphate; N/A, not available; NADH, nicotinamide adenine dinucleotide.