Figure 4.

Effects of the menstrual cycle and pregnancy on CBS and CSE protein localization in human UAs. Uterine artery sections were labeled with primary antibodies against CBS or CSE, followed by secondary Alex⁴⁸⁶ (green)-labeled secondary antibody. Endothelial cells were labeled with marker CD31 followed by Alex⁵⁸⁶ (red)-labeled secondary antibody and cell nuclei were stained with DAPI (blue). Fluorescence images were captured for analyzing relative green fluorescence intensity (RFI) to quantify CBS or CSE proteins. pPRM and sPRM: proliferative and secretory phases of the menstrual cycle; P: pregnancy. Representative outlines of borders between UA intima and media were indicated, and UA lumen (l), intima (i), and media (m) were denoted (second panel). Data (mean ± SEM) are from 10 different subjects/group. Bars with different letters differ significantly among the groups (P < 0.05). Scale bar = 25 um.