Figure 4.
5-Aza restored hypoxia-induced KCNMB1 gene repression in uterine arteries. Uterine arteries were isolated from low-altitude pregnant animals and were treated ex vivo with 21.0% O2 (control) and 10.5% O2 (hypoxia) for 48 h in the absence or presence of 5-Aza (10.0 μM). (A) BKCa channel beta-1 subunit (BKβ1) mRNA abundance determined with real-time RT-PCR. (B) BKCa channel beta-1 subunit (BKβ1) protein abundance determined with western blot. Data are means ± SEM from six and five animals for mRNA and protein measurements, respectively. *P < 0.05, hypoxia vs. control.