Figure 5.
5-Aza recovered hypoxia-induced reduction of BKCa channel activity in uterine arteries. Uterine arteries were isolated from low-altitude pregnant animals and were treated ex vivo with 21.0% O2 and 10.5% O2 for 48 h in the absence (A) or presence (B) of 5-Aza (10.0 μM). Arterial myocytes were freshly isolated from uterine arteries, and BKCa channel current density was determined in the absence and presence of tetraethylammonium (TEA, 1.0 mM) as described in Materials and Methods. Data are mean ± SEM of 7 to 10 cells from five animals of each group. *P < 0.05, 10.5% O2 vs. 21.0% O2.