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. Author manuscript; available in PMC: 2019 Feb 7.
Published in final edited form as: Cell Rep. 2019 Jan 15;26(3):720–732.e4. doi: 10.1016/j.celrep.2018.12.076

Figure 4. AVC Development Shows Disruption in tet2/3DM Larvae.

Figure 4.

(A) GFP-labeled AVC endocardium represents Wnt activity in sibling heart, but not tet2/3DM heart. Hearts were dissected from 48-hpf larvae carrying the Tg(7xTCF-Xla.Siam:GFP) transgene. White arrows indicate AVC endocardial cells with Wnt activity.

(B) WISH forAVC markers bmp4 and has2 at 48 hpf.

(C) GFP-labeled endocardium represents AV valve formation in 4-dpf sibling, but not tet2/3DM larvae carrying the Tg(kdrl:EGFP) transgene. White arrows indicate the AV valve. Bottom left images show higher magnification views of AV valve regions.

(D) WISH for AVC marker bmp4 in 48 hpf and confocal imaging for GFP-labeled PE and epicardium in 72-hpf control and IWR-1-treated larvae. Scale bars: 50 μm.