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. 2019 Jan 7;9(2):1503–1513. doi: 10.1021/acscatal.8b04299

Figure 3.

Figure 3

Progress curves of Michael-type additions catalyzed by different 4-OT variants. (a) Addition of 1 (150 mM) to 2a (5 mM) in 20 mM sodium phosphate buffer (pH 6.5) and 5% (v/v) DMSO. Different 4-OT mutants were used as a catalyst at a concentration of 100 μM. YA: 4-OT M45Y/F50A, FYA: 4-OT L8F/M45Y/F50A, WYYA: 4-OT I2W/L8Y/M45Y/F50A, MYYA: 4-OT I2M/L8Y/M45Y/F50A, YYA: 4-OT L8Y/M45Y/F50A. (b) Addition of 1 (65 mM) to 2c (1.3 mM) in 20 mM sodium phosphate buffer (pH 6.5) and 45% (v/v) DMSO. Different 4-OT mutants were used as a catalyst at a concentration of 18 μM. YA: 4-OT M45Y/F50A, YYA: 4-OT L8Y/M45Y/F50A.