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. 2019 Jan 28;15(1):e1007543. doi: 10.1371/journal.ppat.1007543

Fig 4. MCPyV LT and 57kT bind Fbw7α independently of its WD40 domain and MCPyV LT S239.

Fig 4

(A, B) MCPyV LT was pulled-down with XT10 from whole cell lysates of 293A cells transfected with individual or combinations of full-length MCPyV LT S239A (5μg), MCPyV LT-t (10.5μg), MCPyV 57kT (5μg), Fbw7 (4.5μg), ΔFbox (3μg), or R505L (3μg), and immunoblotted with anti-FLAG to detect co-immunoprecipitated Fbw7. (B) A plasmid encoding only the sequence of MCPyV 57kT was similarly pulled-down and co-immunoprecipitated Fbw7 was detected. Asterisks (*) denote non-specific bands.