Table 1. Genes, primer information and accession numbers.

Symbol	Gene name	Rn	Rp	Rt	Primers (5'-3')	Tm (°C)	Fragment size (pb)	Accession numbers
CYTB	Cytochrome b**	6	5	8	R: GCW CCA ATT CAR GTT ART AA F: GGA CGW GGW ATT TAT TAT GGA TC	50	659	MH704746—MH704764
ND4	NADH dehydrogenase 4**	5	5	15	F: TAA TTC GTT GTC ATG GTA ATG F: TCA ACA TGA GCC CTT GGA AG	53	560	MH704765—MH704779
PCB	Putative chitin binding peritrophin-a domain protein	8	5	5	R: CAC TAC GGG TCG TGA AGG TT F: ACA TCC TTG GCC ACA AGA AC	55	757	MH704780—MH704797
TOPO	DNA topoisomerase	5	6	5	F: CAA CAC TTG TAA CCC GAG CA F: ATC ATT GGC CGC ATC TTT AG	56	604	MH704798—MH704813
URO	Uroporphyrinogen decarboxylase	11	6	6	R: TTA AGG GCA GCA AGA GGA GA F: AAC ACA TTT CCT GGC CAA AG	54	563	MH704814—MH704828
ZNFP	Toll-like-2. Transmembrane receptor with TIR domain binding	5	5	5	F: TCC TTG CGG TAA TGA TGT GA F: CTC GAA TGG TGT ACG TGG TG	54	588	MH704829—MH704852

Gene IDs correspond to those in the Rhodnius genome GFF file annotation.

**Published before. Rn: R. neglectus; Rp: R. prolixus; Rt: R. taquarussuensis