Table 2. Nuclear markers (single copy exons) designed in this study.
| Gen | Annotation in the R. prolixus genome | Region amplified | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene ID | Scaffold | Strand | Start | End | Size (bp) | Location | Start | End | ||
| ZNFP | RPRC009262-RA | Tl-like-2: Toll-like-2. Transmembrane receptor with TIR domain binding | KQ034161 | + | 481476 | 486977 | 5501 | Exon 1 | 481599 | 482146 |
| URO | RPRC013534-RA | UROD: Uroporphyrinogen decarboxylase | KQ034105 | - | 970351 | 971418 | 1067 | Exon 1 | 970699 | 971261 |
| TOPO | RPRC012703-RA | DNA topoisomerase | KQ034259 | + | 391034 | 406927 | 15893 | Exon 3 | 404730 | 405333 |
| PCB | RPRC001863-RA | Putative chitin binding peritrophin-a | KQ034056 | + | 8334541 | 8342490 | 7949 | Exon 3 | 8335296 | 8336052 |
Gene IDs correspond to those in the Rhodnius genome GFF file annotation.